Nonselective Adsorbtion of
DNA on Carrier Beads
3.5.21.
Fluorescence response curves obtained in separate experiments show superimposed response curves for samples of b-DNA, DNA and Oligreen injected on Avidin and Streptavidin Sepharose 4B beads. On Avidin almost all injected DNA was nonselectively captured. Only Streptavidin beads from Zymed allowed separation of DNA from b-DNA. Also adsorption of Oligreen on beds was lowest on Zymed Streptavidine beads.
Carrier solution: Tris-EDTA (TE) buffer with 0.01% Tween-20 Concentration of biotinylated DNA: 0.2 micrograms / mL Concentration of non-biotinylated DNA:2 micrograms / mL Sample injection volume: 10 microliters Sample application flow rate: 1 microliter / sec OliGreen injection volume: 10 microliters OliGreen application flow rate: 1 microliter / sec
